EcoR1

In molecular biology, EcoR1 (sometimes spelled with a roman one as in EcoRI) is a commonly used restriction enzyme. It creates sticky ends with 3 base overhangs. The nucleic acid sequence where the enzyme cuts is GAA-TTC, which is a palindrome as the complementary sequence is CTT-AAG.

This restriction enzyme is often used to cut open a plasmid vector to insert a gene-of-interest during gene cloning.