Thomas Horton (died 1649) was an English soldier and Regicide.
Thomas Horton was a soldier in the Parliamentary army during the English Civil War. Though of humble background, he had become a colonel by 1643. His troops played a decisive part in several important engagements, and he was given lands confiscated from a royalist as a reward for his victories. Horton was a commissioner of the high court of justice in 1649, and thus was among those who signed the warrant for the execution of King Charles I of England. Later that year, he died of natural causes while serving with Cromwell in Ireland. His heirs were deprived of their estate at the Restoration.