Triparental mating is used for the introduction of plasmids
into plant-pathogenic bacteria for transformation.
It requires three bacterial strains:
- a helper strain, carrying the genes that code for conjugation and DNA transfer,
- a donor strain, carrying the plasmid and
- a new bacterial strain to introduce the new gene into
Five to seven days are required to determine if the
plasmid was successfully introduced into the new bacterial
strain and confirm that there is no carryover of the helper or
In contrast, Electroporation does not require a helper or
donor strain. This helps
avoid possible contamination with other strains. The introduction
of the plasmid can be verified in the recipient
strain in two days, making electroporation a faster and
more efficient method of transformation.